Data were coded initially by GH and the coding frame, together with the subthemes, independently scrutinised by SP to determine their robustness.
While they often are a source of mutations by insertion into the coding frame of genes, their characteristic long terminal repeat regions allow them to be alternatively spliced.
35 Inter-rater coding reliability was high; LS identified only minor inconsistencies in coding and resolved them iteratively by reference to the coding frame and in discussion with RH. 37 To ensure open discussion of data collection and analysis throughout the project, the project team had monthly conference calls and attended three face to face meetings a year in South Africa.
After double coding each transcript was discussed by the two team members and the coding frame and themes were further refined by examining regularities, convergences and divergences in the data.
In order to ensure the reliability of the qualitative analysis, the application of the coding frame by independent researchers will be periodically cross checked to establish a degree of uniformity.
This allowed us to align the newly assembled contigs against the seven reference CDSs from Ensembl, while respecting the coding frame by inserting frameshifts and stop codons in assembled contigs where needed.
The two reviewers discussed the codings, and the first reviewer re-examined the entire set of findings again, paying attention in particular to the new codes identified by the 2nd reviewer, updating the coding frame where they agreed with interpretations.
However, we did establish reliability of the coding frame by comparing the results of coding of the main rater to an independent assessor for one full workshop (two sessions), and a random check of 10% of the remaining coding by the independent researcher confirmed agreement.
The coding frame underpinned by NPT developed for data analysis of the stroke literature is shown in Table 1.
The balance of sense and antisense codons is close to the equilibrium, which justifies an error rate of ~15% on the coding frame diagnosis by Rrr.
