Sentence examples for by the clamp loader from inspiring English sources

PCNA is loaded on the P/T junction of a nascent Okazaki fragment by the clamp loader RFC.

PCNA is loaded onto primer-template pDNA by the clamp loader RFC, a five-subunit complex that performs mechanical work through ATP binding and hydrolysis27,28,29,30.

The primer/template junction is recognized by the clamp loader, which loads the clamp onto this DNA structure.

Recruitment of TopBP1 depends on the RAD9/RAD1/HUS1 (9.1.1) complex that is, in turn, loaded by the clamp loader RAD17/RFC2-5 RAD17/RFC2-5

5 This process stimulates the hydrolysis of up to three ATP molecules by the clamp loader to enable closing of the clamp to encircle the DNA and subsequent dissociation of the loader.

The model in Figure 6 also shows how the binding of primed DNA by the clamp loader would automatically position the duplex region of a primer-template junction through the ring of the sliding clamp.

The collar of the clamp loader is replaced by PCNA, so that DNA bound by the ATPase subunits (shades of blue) can thread through PCNA.

The ATPase activity of the clamp loader is stimulated by binding both to the clamp and to DNA [ 39, 40], and upon ATP hydrolysis the affinity of the clamp loader for both clamp and DNA is greatly diminished, leading to ejection of the clamp from the clamp loader.

The other four positions in the clamp loader are occupied by similarly sized proteins: RFC4 at B, RFC3 at C, RFC2 at D, and the ATPase-incompetent RFC5 protein at the E position.

Thus, these residues, which we show take part in the binding of human PCNA to DNA, may play a role in clamp loading by guiding DNA through the open clamp and into the clamp loader to form a tight complex.

The duplex segment of primer-template DNA is positioned in the central chamber of the clamp loader where it is stabilized by basic residues and amino-terminal helix dipoles lining the interior surface of the clamp loader spiral.