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Our results show that on the behavioral level, the period length and strength of the circadian rhythm are significantly modulated by the circalunar clock.
We hence conclude that the overall transcript levels of clock, period, pdp1, and timeless are directly or indirectly modulated by the circalunar clock.
Whereas our behavioral analyses focused on premature adult Platynereis worms, we propose that the observed modulation of circadian behavior by the circalunar clock also underlies the regulation of other behaviors, such as the nuptial dance of mature animals.
We therefore next investigated if the oscillation and levels of circadian clock gene orthologs were also affected by the circalunar clock by comparing RNA levels between NM and FR-FM (cf. Figure 1C).
Remarkably, we see the same genes upregulated by the circalunar clock in Platynereis, raising the possibility that, in analogy to Drosophila, an increase in RNA levels of Platynereis clock can account for the upregulation of period, pdp1, and timeless transcript levels, and in consequence for the significant shortening of the circadian behavioral period length of the worm.
Similar(55)
We started by comparing the Platynereis circadian locomotor activity cycles between two different phases of the circalunar clock (NM versus FR-FM; see Figure 1C).
Live readouts of the circalunar clock in individual worms will be helpful to answer such and further questions on circalunar and circadian clock interactions in the future.
This provides strong evidence that the circalunar clock affects circadian behavior.
This establishes changes in RNA levels as a direct or indirect output of the circalunar clock.
Whereas the circalunar-clock-controlled reproductive timing rhythms are insensitive to the functional disruption of circadian clock gene oscillations, the circalunar clock affects the circadian clock on at least two levels.
It is for instance conceivable that the circalunar clock affects the levels of hormone precursors, processing enzymes or neurotransmitters.
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