Sentence examples for by the boiling method from inspiring English sources

DNA templates were prepared by the boiling method.

DNA was extracted from a fresh subculture by the boiling method.

DNA for amplification was extracted from whole cells by the boiling method as follows.

The supernatant was removed, cells were washed using NS, and lysates were prepared by the boiling method.

LAMP assays were performed using cell numbers ranging from 0 to 1000 cells/reaction and DNA template was prepared by the boiling method as described above.

We extracted DNA from the strain by using the boiling method.

Genomic DNA was extracted from dilution tubes considered for MPN enumeration by comparing two methods: the DNeasy Blood and Tissue kit (Qiagen, Milano, IT) according to the manufacturer's instructions and the boiling method reported by de Oliveira et al. [ 16].

The simultaneous detection of E. coli, Salmonella spp. and S. aureus was developed on a rapid, convenient and sensitive method consisting of selective co-enrichment in ESS-3 broth, DNA extraction with the boiling method and robust test by multiplex PCR.

Two methods were used for every isolate examined: the boiling method (14) and alkaline lysis (15).

Campylobacter spp. DNA was extracted from presumptive colonies using the boiling method.

DNA template for all bacterial strains was prepared using the boiling method [ 16].