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Part of it may be explained by the biological differences between men and women.
The differences introduced by RNA degradation were largely outweighed by the biological differences between the patients.
Although this result might have been caused by the biological differences between pGBM and adult cancers or by the pGBM CSC properties, our intriguing finding may also reflect the complex nature of gene expression regulations in human cancers.
Other experiments at our facility using the more recent Illumina Human HT-12 and Mouse Ref 8 BeadChips exhibit similar batch effects to those in this study; samples are observed to cluster preferentially with others processed in the same run, rather than by the biological differences between them, even after quantile normalisation (data not shown).
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This finding is logically explained by the biological difference between the samples compared in the two studies: in the present study, we compared a growing HepG2 cell line with a stationary liver biopsy sample, while Costantini et al. [ 13] compared two cell lines, each dividing at a different rate; thus, these genes were not differentially expressed.
As an additional result from GSNCA, we define hub genes as genes with the largest weights and show that these genes correspond frequently to major and specific pathway regulators, as well as to genes that are most affected by the biological difference between two conditions.
The differences can be explained by the different experimental protocols and the biological differences between the nude and SCID mice.
We demonstrated our approach by using it to investigate the biological differences in two soft tissue sarcomas, GIST and LMS.
These analyses confirm the idea that, although the phage genome DNA sequences are syntenic, significant changes have occurred between every member of this sub-family of phages, which is also reflected in the biological differences exhibited by phages DLP1 and DLP2.
Vilain's research has suggested that the biological differences between men and women may depend on more than hormonal differences.
The set of individual differentially expressed genes can only tell us which genes are altered by biological differences between different cell types and/or states.
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