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The N-terminal membrane anchor and basic regions of P450c21 were replaced by the basic region of CYP2C3.
Specificity of DNA binding has been predicted to be imparted by the basic region.
As previously described [6], with the parent HeLa cells depleted of endogenous HCF-1, 40% of cells were able to pass from the G1 to S phase and this level was not altered by the Basic region deletion mutant HCF-1 NΔBasic (Fig. 4B).
In this study, we demonstrate using EGFP fusion constructs that KLF6/KLF6-SV1 nucleo-cytoplasmic transport is not regulated by the 5' basic region but activated by a novel NLS encoded within the ZF domain, and a nuclear export signal (NES) located in the first 16 amino acids of the shared N-terminus sequence.
The DNA binding activity of bHLH proteins is mainly determined by the basic region [ 2].
DNA-binding is mediated by the basic region and requires dimer formation of two AP-2 proteins via the helix-span-helix motifs.
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The novel bZIP classes are characterized by substitutions in residues within the basic region that are typically conserved in other organisms, and may play an important role in DNA-binding specificity.
Of the remaining eight, in Glyma12g04933.1 (GmbZIP80) and Phvul.011G047100.1 (PvbZIP67), the conserved Asn (N) in the basic region was replaced by Lys (K).
In Glyma03g35101.1 (Glyma19g37801.1a19g37801.1 (GMedtr7g104190.1r7g104190.1 (MtbZIP52) and Ca_00780 (CabZIP15), the conserved Arg/Lys (R/K) in the basic region was substituted by an Ile (I), whereas in Glyma11g28880.1 (GmbZIP75), the conserved Arg/Lys (R/K) in the basic region was substituted by a Trp (W).
The basic region and leucine repeats are indicated by the bar and arrows, respectively.
And the basic region and leucine zipper coiled-coil motif region was linked by a hinge region.
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