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Timing of firing at origins is approximated by the average replication timing of the peak loci.
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Whereas cyclin E expression by itself decreased the average replication rate from 1.18 Kb/min in cells expressing an empty vector to 0.79 Kb/min (P < 2.4 × 10−21), folate deficiency further reduced the average replication rate to 0.59 Kb/min (P < 1 × 10−13) (Fig 3A).
The average replication time for all probes overlapping every locus was calculated by "Group" command in "Join, Subtract and Group" menu.
The average replication timing T50 varied slightly in different chromosomes.
Chromosomal locations of origins were approximated by the peaks identified using the Peakfinder software [25] based on the profiles of the average replication timing T50 in cds1Δ cells.
The average replication timings T50 of the efficient-replication regions appeared to be remarkably later than the T50 of the inefficient regions p-value<10−15 p-value<10−15.
1- Data derived from the average replication time from two synchronizations.
We found that for a large part of the genome the elongation time can be well described by a global average replication rate, thus by a single parameter.
Peaks identified from the profiles of average replication timing T50 were commonly used to predict the location of replication origins (or origin clusters).
Fold induction is calculated by dividing the average expression from metaphase I anaphase I by the average expression from DNA replication-prophase I. (C ) Ordered plot for mRNA-seq and ribosome footprinting data for NDC80 and (D ) HSK3 at the indicated stages.
The transcript level of each EST was represented by the average over six measures, including dye swap replication and three in-slide replications within either Cy3 or Cy5 replications.
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