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The efficacy of ECC was evaluated by the average depth and the percentage of ECC with adequate depth; 39.8 ± 8.2 mm and 46.8 ± 48.8% on the floor and 34.4 ± 6.9 mm and 41.7 ± 42.7% in the dental chair.
The relative ribosome density at a specific position was calculated by dividing the read depth of the position by the average depth of 121 bases (60 nts to the 5′ and 3′ directions), and the average depth of each codon located at the A-site (position 0) was calculated using all CDS reads.
To calculate the volume of your pond, multiply the number of surface acres by the average depth of the pond in feet.
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Spawning depth for each individual was estimated by finding the average depth over which the gill nets were set during each sampling event.
By maintaining the average depth of coverage around 1000X to 2000X the number of bases with low (50X to 100X) coverage can be minimized.
To compare the read depth of each genome position, mapping information of each read was used to summarize the read depth at each genome position of each strand, and the data were normalized between samples by defining the average depth of the total coding region as one.
For the 20 genes where testing is currently available in our laboratory by Sanger sequencing, the average depth of coverage was 272 and 99.4% of bases had a minimum read depth of 30 (Fig. 1a).
As determined by SAMtools [ 34], the average depth of the generated P. notoginseng transcriptomes was over 130 × .
For example, if the pool has a minimum depth of 5 feet and a maximum depth of 10 feet, then you can add 5 + 10 to get 15, and divide 15 by 2 to get 7.5, the average depth of the pool.
The relative depth of a genome position was calculated by normalizing the depth on the position according to the average depth from -60 to +60 nts.
For a given putatively association region i, and for each sample, k, we calculate { raijk} as the average depth in non-overlapping 100-bp windows j divided by the genome-wide average depth.
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