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If the control results were outside of the acceptable limits determined by the assay method validation, the run was rejected, and all samples on the same gel were repeated.
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We genotyped these SNPs by the TaqMan® assay method using the ABI 7900 DNA detection system (Applied Biosystems, Foster City, California).
The total protein content of the resulting supernatant was determined by the Bradford assay method.
Total protein concentration of the samples was determined by the Bradford assay method (Bradford 1976).
The virus was quantified by the plaque assay method of Wentworth and French [77] with MEFs, as reported previously [78].
The concentration of total soluble protein was estimated by the Bradford assay method.
The cytotoxicity of CCNBs against the human embryonic kidney (HEK) 293T cells was tested by the MTT assay method.
Hence, RBCs are frequently used in fish to evaluate DNA damage by the comet assay method.
As regards this study, EVs were quantified by the plaque assay method in confluent monolayers of BGM cells grown in cell culture plates (Bae and Schwab 2008).
Protein concentration was determined by the Bradford assay method (Bio-Rad).
Protein concentration was determined by the Bradford assay method (Bio-Rad, Hercules, CA).
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