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The force sensing ranges can be tuned by varying frequency of the driving voltage supplied by the array scanning circuitry.
Here, W represents the range of possible values for observations, i.e. all positive real-valued intensities generated by the array scanning software (in our case [0, 2 − 1]).
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The photostability of HyPer5 is further documented by repeat array scanning without loss of detection.
After washing and drying the slide by centrifugation, the arrays were scanned with a High-Resolution Microarray C Scanner (Agilent Technologies) with a resolution of 3 μm.
cDNA production and labeling, hybridization and array scanning were carried out by MOgene (St . Louis MO), using the DNase I-digested RNA.
After washing and staining with streptavidin-phycoerythrin (Invitrogen) by GeneChip Fluidics Station 450XP (Affymetrix), the array was scanned by a GeneChip Scanner 3000 (Affymetrix).
Hybridized probes were captured by streptavidin phycoerythrin conjugates, and the array was scanned and genotypes identified.
Hybridization and array scanning were performed by Roche NimbleGen as previously described [ 39].
The labeled DNA fragments were hybridized to the microarray, the hybridized DNA probes were captured by streptavidin-phycoerythrin conjugates, and the array was scanned.
After washing and drying, the array was scanned by ScanArray Express Microarray Scanner Packard Bioscience Biochip Technologyy, PerkinElmer) at a wavelength of 633 nm.
Hybridization is detected by incubation with streptavidin-phycoerythrin conjugates, followed by scanning the array for phycoerythrin fluorescence and quantitation.
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