The results also demonstrate the superior robustness of the method by repeating the analyses using random experimental designs.
The results obtained by repeating the analyses using random effects models were highly consistent with the main findings (web table 5).
We also repeated the analyses using estimation by Generalised Estimating Equations.
We have validated the Dam-seq method by the following analyses using the data from 3T3 MEFs.
We used quantitative PCR to validate the expression of some of the genes identified by the microarray analyses using RNA from individual animals from the four groups.
In addition to the Mj 7.0 event, additional crustal deformation is detected by the InSAR analyses using different SAR data pairs.
The proteins in the gradient were detected by Western analyses using the indicated antibodies.
The gradient was fractionated, TCA precipitated and the protein content was assessed by Western analyses using the indicated antibodies.
The silencing efficiency was detected by immunoblot analyses using the anti-ubiquitin antibody.
The topological stability was confirmed by phylogenetic analyses using the JTT model [33].
