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To our knowledge, the bottom of the each concave microlens is affected by the alignment error (around 1 μm) between the vertex of the cutting edge and the spindle axis.
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For this reason, we assess the performance of the algorithm in terms of effectiveness by measuring the alignment error for different free-form objects.
To reduce the possibility that the initial EST test might have been affected by the alignment errors introduced by the methods in their mRNA alignments and repeated in the EST alignments, we repeated the test with an extra requirement that the set of introns must be the same in the mRNA alignments produced by every method.
(The number of images required and the alignment error are not completely independent, and it is possible to make up for alignment errors by collecting more images).
The signal-to-noise ratio also influences the alignment error.
These differences can be improved by correcting the alignment errors of the MAST.
All of which strongly suggests that Dr Spence's method may indeed have been the one used to align the pyramids.Furthermore, by working backwards from the alignment errors, this theory can be used to provide more accurate estimates of the dates of the pyramids' construction.
In order to evaluate the robustness against the alignment errors, we realigned every alignment by ClustalW (Thompson et al., 1994), which considers no structural information such as covariation.
The quality of the detected translocations using NGS data may be affected by the NGS alignment errors and duplicate callings.
After that, the final tool path is modified by considering alignment error, tool radius error and squareness error.
To control for bias introduced by alignment error or by other technical sources, genomic DNA from the same F1 genotype was collected ([ 48], SRA accession number SRA048616).
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