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Under these conditions, we analysed cell responsiveness to N-activation by testing the expression of the fate determinant Tramtrack.
We therefore analysed RPE differentiation by testing the expression of an RPE-specific marker, RPE65, five days after injection of LNT.shGFP, LNT.ZONAB or LNT.shZO-1 [18].
We verified the results by testing the expression of PSMB6 and EEF1A1 at five developmental stages in oocytes (GV, MI, MII) and embryos (D2, D3, D5).
We first checked that the mouse model was working appropriately by testing the expression of the two Mecp2 isoforms described to date [7], [26].
The authors went on to confirm this conclusion by testing the expression of two reporter genes in Escherichia coli.
The latter possibility was experimentally excluded by testing the expression of a plasmid-borne IND1 p.N271QfsX31 in the heterozygous strain ind1Δ /+.
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The fourth problem (incorrect specification of the regular expressions) can be addressed by testing the regular expression definition against some sample data using publicly available tools such as http://www.regextester.com/.
We further confirmed our model by testing the gene expression level of iNOS and p53 and p53 dependent target genes in VP16 or VP16-SXR transfected MCF-7 cells.
The list of genes matching each function is shown in Additional file 3. Validation of the microarray results was performed by testing the relative expression of 16 genes (see Additional file 4) in the same RNA samples that were used for the chip hybridization, by real-time PCR.
In bone tissue the most abundant connexin isoform is Cx43 [ 11], which could be confirmed by relative quantification PCR, testing the expression of Cx37, Cx40, and Cx43 in the present coculture model system.
CNS-specific gene expression was evaluated by testing the distribution of expression levels in the CNS (11 tissues) against the distribution of expression levels across all tissues included in GeneAtlas (two-sided Student's t-test).
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by testing the productivity
by testing the art
by testing the weight
by testing the engine
by testing the strength
by testing the distance
by testing the ability
by testing the average
by testing the flywheel
by testing the adsorption
by substituting the expression
by testing the significance
by testing the differentiation
by testing the model
by testing the authenticity
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