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In our study, crude amylase was heated at different temperatures for 1 h followed by testing its activity.
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The determined most effective ionic solution (tetra alkyl ammonium sulfate known as Ammoeng 102) was further investigated by testing its surface activity at various conditions of pressure, temperature and solution salinity.
We expressed the NDPK from S.cerevisiae and tested its activity by titrating GTP in the two reaction buffers using the GTPase-Glo reagent.
We previously identified CPTH6 as a new inhibitor of histone acetyltransferase activity, and by testing its effect on α-tubulin, a decrease in acetylation level of the protein was observed in U-937 cells and H1299 after 48 h of treatment; in contrast, CPTH6 treatment did not modulate the expression of HDAC6 deacetylase.
We then determined the actual angiogenesis activity of each compound by testing its dose dependent response directly in the cord formation assay.
The chemical properties essential for bactericidal activity against Escherichia coli were probed by testing antibacterial activity in the presence of metal chelators, the hydroxyl radical scavenger, thiourea, and varying pH levels.
This was confirmed by testing the activity of a mutant form of the p391 promoter (p391 del (PEA)) that contains a short deletion in one of the PEA3 motifs (the −122 bp Ets binding site) (Figure 9C).
Conditions for optimal gene expression were established by testing luciferase activity in the skin 48 h following injection of pHCMVIE1-luciferase.
By testing promoter activity of ten plant-specific promoters in five eubacterial species we could show that in 50%% of the tested combinations the promoter sequences could be used in the bacterial host [ 12, 13].
The most potent compound 14c was identified with an IC50 value of 0.10 μM by testing the inhibitory activity against recombinant human FBPase.
In Anopheles stephensi, constitutive and blood induced trypsins were identified by testing the enzymatic activity of midgut extracts on synthetic substrates, and by western blot analysis [30] [32].
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