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We checked the hypothesis of a cation chelation by testing increasing concentrations of EDTA on MCF-7 cell viability and apoptosis.
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The specific, in vivo cytotoxicity of Bi-PAI2 against the uPA receptor was directly tested by preinjection of increasing concentrations of PAI2, before Bi-PAI2 administration.
To test pulmonary endothelial dysfunction, bradykinin (BK -induced vasoconstriction was evaluated BK -inducedg increasing concentrations of BK (1, 3, and 6 μg; Sigma Chemicals) into the inflow circuit.
The relaxation relative to PGF2α induced tone in human meningeal arteries by increasing concentrations of each of the tested substances (A-F) and NaCl buffer (G).
During the calcium fluorescence assay, cells were incubated with vehicle or test compound at a fixed concentration of 30 μM, followed by increasing concentrations of glutamate.
Next, we tested the effects of increasing concentrations of Stl on the hydrolysis of the phosphodiester bond, a reaction also catalyzed by the RNAP active center.
Phosphate-buffered saline solution was inhaled first, followed by test solution in two-fold increasing concentrations (0.06 to 16 mg/ml).
To determine the maximum level of heart rate decrease that could be induced by inhibition of BK channels, increasing concentrations of paxilline were tested using a new heart for each trial at each concentration.
We also tested the effect of copper depletion on respiratory growth, by culturing yeast in increasing concentrations (1 – 1000 μM) of the copper chelator, bathocuproine disulphonic acid (BCS).
We first measured inhibition of DPAP1 by treating parasite lysates for 30 min with increasing concentrations of test compounds followed by labeling of residual DPAP1 activity with FY01.
The concentrations of tested compounds that inhibited the hydrolysis of substrates (acetylthiocholine and butyrylthiocholine) by 50% (IC50) were determined by monitoring the effect of increasing concentrations of these compounds in the assays on the inhibition values.
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