Sentence examples for by testing different conditions from inspiring English sources
However, to further decrease the residual phosphorylation of PtdIns4 P, which could cause a bias of quantification since it is much more abundant in mammalian cells than PtdIns3 P, we optimized the assay by testing different conditions, especially variation of ions concentrations and lipid vesicle composition.
By further optimization of ligand structure, testing different conditions and biosensor platforms, this assay has the potential to be translated to rapid diagnostic tests.
Optimized conditions were designed by testing different experimental conditions on model substrates (cyclopentylmethanol, cyclohexylmethanol, cyclopentane-1,1-diyldimethanol or cyclohexane-1,1-diyldimethanol) to find best organic solvent, optimal acyl donor and temperature, as well as the optimal substrates/enzyme ratio.
We previously reported two dysferlin specific llama heavy chain antibody fragments (HCAb) that can specifically immunoprecipitate dysferlin from human skeletal muscle.[23] We adapted the immunoprecipitation method for cultured cells by testing different buffer conditions (Figure 2).
We tested different conditions with DNA polymerases of various sources.
We have also explored various PCR amplification conditions by testing different polymerases, and their tolerance to AT-rich templates, in the absence or presence of tetramethylammonium chloride (TMAC), a PCR additive that increases thermostability of AT base pairs [ 7].
By the test facility, entrainment ratio and hydrogen mole recirculation ratio of the designed ejector were tested on different conditions, corresponding to the PEMFC stack operation protocols.
The TEV protease cleavage site was tested under different conditions.
We tested different isolation methods and culture conditions and validated purity of the cultured cells by immunohistochemistry against keratins.
We tested different strains of P. acnes grown under different conditions and could not detect any EPS-like structure by EM analyses (data not shown).
These conditions have been validated by simulation tests under different conditions of temperature and irradiance.
