Sentence examples for by targeted integration using from inspiring English sources

pUASp-attB-vilya 3XHA was introduced into Drosophila by targeted integration using the attP-40 line (Genetic Services, Boston, MA).

When cells containing a docking platform marked by GFP expression are challenged with a plasmid that contains a MTX-resistance marker between two attB sites, in combination with an integrase-expressing plasmid, correctly integrated products should be resistant to MTX and should fail to express GFP; we initially tried to select targeted integration using either or both of these properties.

As our lab is interested in pursuing targeted integration using active 'cryptic' sites, we searched the Arabidopsis thaliana genome for the presence of sequences similar to the Bxb1 att sites.

The OCT1-overexpressing HEK293 cells were generated by targeted chromosomal integration using the Flp-In SystemTM (Life Technologies, Darmstadt, Germany).

However, such potential risks can be eliminated by targeted integration of genetic constructs into a defined locus without disrupting other regions of fungal genomic DNA (Weld et al. 2006).

The adcA knockout strain was generated by targeted integration of the bsr cassette at position 496 of the adcA gene.

First, TbAUK1 was PTP-tagged at its C-terminus and expressed at the endogenous level in procyclic trypanosomes by targeted integration of the PTP vector into an endogenous TbAUK1 allele (Fig. 1A).

Gene disruption by targeted integration of transfected constructs becomes increasingly popular for studies of gene function.

These pitfalls can be circumvented by targeted integration of genetic constructs into a defined locus without disrupting other regions of fungal genomic DNA (Weld et al., 2006).

Similarly, correction of CGD by targeted integration of a therapeutic cassette into the AAVS1 locus was shown to restore superoxide production in granulocytes derived from the targeted iPSC (Zou et al, 2011).

Since the industrial strain TMB3400 was obtained from USM21 not only by targeted integration of the xylose pathway but also by further random mutagenesis, three S. cerevisiae strains derived from the same strain background without mutagenesis, which produce Ps-XR at different levels were used to confirm that Ps-XR was the enzyme responsible for HMF and furfural improved reduction in TMB3400.