Sentence examples for by targeted insertion of from inspiring English sources

The method requires, in its initial stage, to establish the seed mouse by targeted insertion of the landing pad via conventional ES cell-based gene targeting approach.

Srinivas, S. et al. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus.

This pitfall may be overcome by using clonal derivatives of the test cells or by targeted insertion of the recombinase-activatable transcription unit into specific chromosomal loci.

The R26R-EYFP strain (Figure 1C) was generated by targeted insertion of a Cre reporter cassette into the ROSA26 genomic locus, which has been shown to be ubiquitously expressed throughout development and in adult tissues [7], [8].

Furthermore, in the ApoE knock-out murine model of atherosclerosis, Hp2/Hp2 mice obtained by targeted insertion of a murine type 2 Hp allele into the Hp locus by homologous recombination show increased iron deposition, lipid peroxidation and macrophage accumulation within atherosclerotic plaque, increasing the risk of rupture and thrombosis [30].

The generation of a stable Nat2 knockout line of mice by targeted insertion of a lacZ-containing cassette, has been described [11].

76 Meanwhile, vector-mediated genotoxicity has fostered a growing interest for alternative strategies, referred to as "targeted approaches," that consist of replacing random integration of therapeutic transgenes by targeted insertion or even correction of the deleterious mutation.

The transcription unit was introduced by targeted insertion into ES cells by homologous recombination at the hprt locus.

Because the viability of the mutants (GY02) in antifungal drugs might be influenced by 5-FOA, the BPDR5 gene was disrupted in GY02 by targeted insertion with URA3 as the selection marker to reconstruct the pdr5 null mutants (GY03).

Since PCR with primer set 'a' is applicable to identification of targeted insertion of DOI performed by either systems, we used this primer set for the first screening in all i-PITT experiments.

Nakao, H. et al. A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.