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Novel miRNA-target genes were further identified by tandem array analysis.
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The PPR family is characterized by tandem arrays of the PPR motif, a highly degenerate repeat of 35 canonical amino acids (Small and Peeters 2000).
BLAST analysis of MLONG1 indicated that this repeat was in itself a composite unit, built by a tandem array of ten MSHORT1 elements (Additional File 1 Figure S3A).
Our observations indicate that CMITEs can increase their copy numbers not only by transposition, but also by forming tandem arrays.
Its origin was confirmed by tandem repeat analysis (data not shown).
Furthermore, newly reduced products help obtain increased structural information by tandem MS analysis.
For both proteins, the identity of the N-terminal peptide was established by tandem MS analysis.
The peptides were subjected to nanoelectrospray ionization followed by tandem mass analysis.
The comparison of these sequences suggests a simple mechanism by which a single CUP1 gene is duplicated to form a tandem array by unequal non-homologous recombination.
We detected RnaG by both RNAz and tiling array analysis.
Potential centromeric sequences were assessed by finding the monomeric tandem array that covered the largest amount of the genome.
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