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Briefly, by taking equal amounts of protein from each sample, 10 cancer samples and 10 control samples were pooled separately, with each pool containing 250 µg of proteins.
Oligo d(T) primed reverse transcription was performed using Omniscript Reverse Transcriptase (QIAGEN, Mississauga, Canada) in a 20 μl reaction volume (42°C, 1 h) by taking equal amounts of RNA (100 ng) from the Graffi-leukaemic cells and the murine HB22.2 cell line.
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Therefore we took equal amounts of total proteins, electrophoresed them and tested the antibodies by immunoblotting.
For example, it would have to abandon its practice of offsetting its bond purchases by taking in equal amounts in commercial bank deposits.
The 24 samples and a common reference pooled sample (made by taking an equal amount of protein from the EV 12 and EV 24 samples) were placed on eight 4-plex iTRAQ runs as described in Additional file 1.
Arrange your deck to have equal amounts!
If you want to start taking money out before 55, one way to do so penalty-free is by taking "substantially equal payments". You annuitize your 401(k) or IRA taking out an actuarial amount that applies to your age.
Take an equal amount of hair oil and shikakai powder.
Here's how to do it: Take an equal amount of 3% hydrogen peroxide and water.
The two volunteers took turns applying equal amounts of pressure to each other's fingers while the researchers measured the actual amount of pressure they applied.
There, child dependency fell across the board by roughly equal amounts.
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CEO of Professional Science Editing for Scientists @ prosciediting.com