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Using the list of nurses and doctors in each hospital as sampling frames, 50 nurses and 25 doctors were selected from each hospital by systematic sampling to give a total of 100 doctors and 200 nurses for this study.
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In the second stage of sampling, all private households within the cluster were listed (institutions excluded) and an average of 25 houses within a cluster were selected by equal probability systematic sampling to be surveyed.
The health facility sub-samples were chosen by systematic sampling proportional to clinic enrolment size.
Taking this into consideration, a representative sample size of 1,108 subjects (including 10% to correct for non-responders) was selected by systematic sampling with probability-proportionate-to-size from a list of 270 schools from Bucharest.
In the first stage, primary sampling units (PSU) were selected by systematic sampling with probability proportional to the number of households according to the 2000 Census.
Households with eligible study participants were selected by systematic sampling method through house-to-house visits.
Cross-sectional study, 866 participants (53.2% girls, 46.8% boys, age range 6 18 years), selected by systematic sampling with probability-proportionate-to-size from all Bucharest's schools.
Total cell number was obtained according to the optical disector principle, by systematic sampling of counting frames of 15-µm side in each section.
Additionally, in order to check that our results are not affected by systematic sampling bias, we analyzed the same sets of impact trajectories in panels (A-D) using a shuffling method to destroy the author-specific correlations across time.
The first-wave participants were selected by systematic sampling: 30% of subjects were sampled from individuals aged 45 to 64 years and 70% were sampled from individuals aged 65 years or older.
According to the procedure presented, a sampling plan could be considered optimized and complete with 19 sampling stations for this study site (comparatively to 38 sampling stations for the total set obtained by systematic sampling).
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