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Neutrophil activation was assessed by surface expression levels of L-selectin and CD11b.
To further exclude that Trx1 reactivity of receptors is determined or limited by surface expression levels, we ectopically overexpressed CD30 or other TNFR superfamily members under control of the same promoter in HeLa cells and analyzed Trx1 cell surface trapping by indirect immunofluorescence.
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Neutrophil infiltrations in vital organs are not only affected by the circulating levels of inflammatory chemokines such as CXCL1/KC, but also by the surface expression levels of chemokine receptors such as CXCR2.
The preference for CD30 could not be explained by exceptional surface expression levels either.
The reduced Emax values are most likely caused by reduction in cell surface expression levels of these V2R orthologs.
L. lactis expressing each FnBPA construct at three surface expression levels (by inducing expression at three concentrations of nisin; 0, 10 or 100 ng ml−1) were assessed for invasion of HaCat cells (Fig. 6).
As CD133 has been reported to be a CSC marker for colon cancers, we determined CD133 epithelial cell surface expression levels by flow cytometry.
These cells maintained high expression levels of the major collagen integrins, β1, α1 and α2 integrin subunits, as demonstrated by flow cytometry assessment of surface expression levels of these receptors.
Cell surface expression levels were determined by whole cell ELISA using an antibody directed against the N-terminal FLAG-tag of the receptor.
Cell surface expression levels were examined by surface enzyme-linked immunosorbent assay.
Although this difference was statistically significant (P = 0.047 by student t-test), the increase in surface expression levels was subtle.
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