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The wear mechanism has been investigated by surface analyses using cross-sectional transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS).
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By the surface plasmon resonance analyses using a C-terminal fragment of AQP2, high affinity binding of actin was observed, showing that actin itself is one of the AQP2 binding proteins (Noda et al. 2004b).
This was confirmed by quantitative analyses using a CPC assay.
Electrochemical techniques complemented by ATR-FTIR, XPS, SEM and AFM surface analyses were used to evaluate the composition and protective characteristics of the films.
Total cell surface area was analysed using the ZeissAxio Imaging software.
The 11B6 binding kinetics were analysed by surface plasmon resonance using a Biacore 2000 (Biacore AB; Uppsala, Sweden).
MHC II cell surface expression was measured by LSR Fortessa and analysed using FlowJo.
GAM analyses used the MGCV library by Simon Wood, version 1.7-18 1.7-18
Surface antigens of P3 were analysed using flow cytometry.
The expression of selected surface markers on PMN was analysed using flow cytometry.
The surface antigen expression patterns on the UC-MSCs were analysed using flow cytometry.
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