We used a multi-factorial experimental design in which we induced hypothyroidism, followed by supplementation with T2 and T3 (plus normal thyroid controls) in zebrafish exposed to different chronic and acute temperature combinations.
In chronological aging experiments, Δspe1 yeast cells exhibited an increased mortality (and hence a shortened lifespan), which could be restored to normal levels by supplementation with low doses (0.1 mM) of spermidine or its precursor putrescine [ 94].
Other studies have indicated that, under normal conditions, As methylation is not enhanced by supplementation with methyl donors in humans (Buchet et al. 1982) or in animals (Buchet and Lauwerys 1987).
Transgene expression could be suppressed by supplementation with Dox as expected and mice maintained on Dox during embryonic development and in the post-natal period were normal at birth, did not have an epidermal barrier defect and survived to adulthood.
The restoration of normal NO levels in diseased arteries is therefore a major therapeutic goal; this could be achieved by supplementation with exogenous NO or by strategies designed to increase the concentration of endogenous NO.
Rodent studies have also shown that insulin resistance can be reversed by supplementation with fish oil11,12,13.
Under oxidative stress (induced by supplementation with 0.15 mM menadione), the expression profiles of MmTKL1 and MmTKL2 differed considerably.
Physiologic osmolarity was induced by supplementation with 120 mM NaCl [44].
Recovery of cytoplasmic pH was enhanced equally by supplementation with NaCl, KCl, proline, or sucrose.
The K+ requirement however was partly compensated by supplementation with NaCl, choline chloride, proline, or sucrose.
