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A combination of light scattering, transmission electron microscopy, and fluorescence correlation spectroscopy was used to show that neither the size, nor the morphology of the polymersomes was affected by successful insertion of gA in the polymer membrane.
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It is recognized and processed by INT during the successful insertion of the new copy into the host genome [ 26].
The inserted araC-Cre expression cassette and the entire bacterial lacZ::araC-Cre locus were amplified by long-range PCR, thus, proving successful insertion of the araC-Cre cassette into the lacZ gene of the strain HB101.
Successful insertion of eGFP was detected by PCR using primers embedded in the inserted sequence and genomic sequences (Fig. 3A,C; supplementary material Fig. S6).
Clones were tested for successful insertion of artificial CGIs by PCR screen and Southern Blot.
Pools of selected cells were tested for successful insertion of DNA libraries by PCR using primers recognizing the universal priming region flanking the insertion site.
The successful insertion of the exogenous genes was confirmed by PCR with genomic DNA isolated from leaves of independent transformants as templates.
Successful insertion of the th-P2A-Gal4 donor was then verified by PCR and junction sequencing analysis in F1 progenies (Supplementary information, Figures S1B5 and S1B6).
Time from completion of draping to successful insertion of needle.
The parameters studied included Time from completion of draping to successful insertion of needle.
XRD pattern confirmed the successful insertion of copper ions in the lattice structure of Na-Ni-Co-Mn layered oxide.
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