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Immunolabeled cells were analyzed by flow cytometry, and the increased number of protein-expressing cells was determined by subtracting the expression in unexposed cells from exposed cells.
Log2 ratios between tumor and normal samples were calculated by subtracting the expression values (log2-transformed absolute reading) of each tumor sample by the mean expression value of all normal samples included in the dataset.
Differential expression was determined by subtracting the expression value in the non-malignant line MCF10A from the value in each cancer line.
On logarithmized expression data, a correction factor was calculated by subtracting the expression of PNLIPRP2 in a given sample from median PNLIPRP2 expression.
The relative expression (also called fold change) of a gene in a particular tumor is computed by subtracting the expression of the gene in the tumor from the mean expression of the same gene in the reference sample set.
After RMA pre-processing and averaging of replicates, a differential expression time series of each probe set was formed by subtracting the expression values measured in the non-exposed control from the values in the asbestos exposed sample at each time point.
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The expression values were normalized by subtracting the average expression value of a tissue and then adding the average expression value of the whole dataset.
Gene expression values were rescaled as relative gene expression values, calculated by subtracting the mean expression value across samples from the gene estimate and dividing by the standard deviation across patients.
Gene expression is normalized by subtracting the aggregated expression of the reference genes and algorithmically combined to calculate the genomic prostate score (GPS) [ 51].
Gene expression was normalized by subtracting the aggregated expression of the reference genes from the weighted mean Cp for each of the 12 informative genes and centering by adding 10 units to the result.
The difference in expression frequency for each gene between each group was obtained by subtracting the gene expression frequency in PDX from the gene expression frequency in primary tumors.
More suggestions(15)
by subtracting the value
by subtracting the mean
by subtracting the divisor
by subtracting the number
by subtracting the therapy
by substituting the expression
by subtracting the baseline
by subtracting the column-wise
by subtracting the overhead
by subtracting the output
by subtracting the debt
by increasing the expression
by subtracting the rate
by regulating the expression
by subtracting the ΔCt
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