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We tested multiple predictions about the possible role of this binding-site variant in silencing by substituting the native Rap1 binding site at the HMR-E silencer with the genome-wide consensus sequence for Rap1.
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More specifically, it explores the effect on the metabolic patterns in Escherichia coli under anaerobic and aerobic conditions of substituting the native cofactor-independent formate dehydrogenase (FDH) by an NAD+-dependent FDH from Candida boidinii.
L'Equipe claim Domenech responded by substituting the player.
By substituting the joint m.g.f.
I made a "breakfast" version of the gruel by substituting the tuna with a fried egg.
NRT works by substituting the nicotine delivered by smoking cigarettes.
This enabled mutagenised replacement DNAs to substitute the native gene and a screen to be performed to find ts mutants.
It has been suggested that the negative effects produced by substituting a native cytoplasm with a cytoplasm from a more or less related species are dependent on the evolutionary distance between the two species [ 4, 13].
DOI: http://dx.doi.org/10.7554/eLife.07956.004 Enhancer 'deletions' were created by substituting native sequences with neutral sequences of similar sizes (see 'Materials and methods').
This element substituted the P1/P3 native promoters of RSF1010 that transcribe, in particular, the gene of their own repressor MobA [ 15].
Interestingly, normal arrestin-1 binding was not recapitulated in vitro by substituting charged residues for native phosphorylation sites (Ling et al., 2004; Ascano and Robinson, 2006; Ascano et al., 2006).
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CEO of Professional Science Editing for Scientists @ prosciediting.com