Sentence examples for by subsequent steps from inspiring English sources
Incubation with primary antibodies was carried out overnight at 4°C followed by subsequent steps as follows: For pro SP-C/Ki67 co-staining sections were incubated with donkey anti-mouse biotinylated antibody (1∶200) and donkey anti rabbit Cy5 antibody (1∶200) for 90 min at room temperature (RT) sections were subsequently incubated with streptavidin conjugated Alexa 555 (1∶200).
However, a sparser sampling of both constructs randomised simultaneously might in some cases allow direct identification of complexes that could be further refined by subsequent steps of rescreening individually, or by limited proteolysis and mass spectrometry depending on the requirements of the downstream application.
GAPDH was diluted in 5% BSA in TBST (1 10,000 dilution) and incubated at RT for 2 h followed by subsequent steps as described above.
Such events should be followed by subsequent steps of gene loss due to relaxed selective pressure in already incomplete pathways, leading to an eventual loss of all genes for a particular biosynthesis pathway in some clades.
The HQ can then be regenerated from aminophenol (AP) by subsequent steps of oxidation, followed by the hydrolysis of imine to generate BQ with the liberation of ammonia and then further reduction of BQ to HQ through a classic EC (electrode and chemical) mechanism.
The first step is always to build up the input file for a geometry optimization, which is followed then by subsequent steps of frequency calculation (freq WF), population analysis (pop WF), time-dependent DFT (TD-DFT, TD WF), and added solvation models (solv WF).
The FR-2 appliances were constructed according to the design recommended by Fränkel and Fränkel [6] with an initial mandibular advancement that did not exceed about 3 mm, followed by subsequent step-by-step advancement of the same entities.
C3b carries out several functions: It brings about lysis (bursting) of the target cell by activating subsequent steps in the cascade, leading to the formation of a ringlike structure called the membrane attack complex.
FRA11A or FRA11H may contribute to setting amplicon boundaries by promoting subsequent steps of BFB cycle.
The maximum force was increased after each testing level, from 500 N in the first stage to 1000 N in the second stage followed by four subsequent steps of 250 N each to a maximum force of 2000 N. The axial excursion of the actuator of the testing machine was recorded at cycle 1, 50 and 100 during each level.
