Sentence examples for by subsequent sequencing of from inspiring English sources
The aim of the work was twofold: firstly to validate a diagnostic real time RT-PCR developed in 2012 for the detection of RHDVb strains and secondly, to design a conventional RT-PCR for the differentiation of RHDVb strains from RHDVb recombinants by subsequent sequencing of the amplicon.
This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region.
To elucidate if any genotypes were predominating among the isolates, mechanisms for FQ resistance were investigated by subsequent sequencing of PCR amplicons.
Confirmation was performed by subsequent sequencing of hemagglutinion, nucleoprotein, or neuraminidase genes by using the BigDye Terminator Cycle Sequencing Quick Start Kit (Beckman Coulter Inc., Krefeld, Germany).
In the case of the PhiYep-3 excision, a single PCR amplification step was sufficient for amplification of the attP attachment site of the circular excised prophage (about 500 bp, proven by subsequent sequencing of the PCR product).
The gene content of the pig bacterial artificial chromosome (BAC) clones predicted on the basis of BES BACC end sequence) alignments with the human genome has been validated by subsequent sequencing of BACs in the pig genome project [ 31], suggesting that the FPC map is a solid tool to identify gene locations.
The significant difference in microbial community structures related to SCFAs production was revealed by subsequent sequencing analysis of straw-conditioning systems and SMS-conditioning systems.
Viruses could be identified by subsequent sequencing and phylogenetic analysis of the amplicons.
Specific skipping of hE50 was confirmed by subsequent sequencing.
However, these findings could not be confirmed by subsequent sequencing.
However, unbiased amplification was confirmed by cloning and subsequent sequencing of cloned the PCR products using genomic DNA from 3D7 as well as from the isolate of the splenectomized patient as a templates (supporting information).
