Sentence examples for by subsequent extraction with from inspiring English sources

Total MeOH extracts of roots and aerial parts of G. urbanum and their fractions obtained by subsequent extraction with petroleum ether, EtOAc and n-BuOH were studied.

The tissue samples were homogenised and total nucleic acids (TNA) prepared by digestion of the homogenate with proteinase K in a SDS-containing buffer, followed by subsequent extraction as previously described [ 21].

Using the post-column dilution of the LC eluent by water and subsequent extraction with the needle interface, the analyte was successfully concentrated to the PDMS phase on the fibrous support in the needle.

DNA from the cream layer of fresh breast milk samples was extracted by using a previously described protocol that included washing with phosphate-buffered saline and subsequent extraction with the DNeasy Blood and Tissue Kit (20 ).

A subsequent extraction with GITC was essential to solubilize the majority of the cellular RNA.

Subsequent extraction with a 5% acetic acid and 5% DS solution of the templating protein from the accessible surface of the MIPPh nanocoatings was manifested by the decrease in electric impedance.

Unmodified 3H-25(OH)D3 and most of the products were totally extracted from the combined solutions plus cell pellet according to the method of Bligh and Dyer [61] by three subsequent extractions with 2, 1 and 1 ml volumes of CHCl3 at room temperature.

Chromatin was prepared by two subsequent extraction steps (10 min at 4°C) with Buffer 1 (50 mM Hepes/KOH pH 7.5; 140 mM NaCl; 1 mM EDTA; 10% glycerol; 0.5% NP-40; 0.25% Triton) and Buffer 2 (200 mM NaCl; 1 mM EDTA; 0.5 mM EGTA; 10 mM Tris pH 8).

Plasma membranes were isolated by differential centrifugation with subsequent extraction of the TRPM8 protein with 1% Nonidet and 0.5% dodecylmaltoside (DDM) (see materials and methods).

Construction of the New River in the seventeenth century to supply drinking water to London, with subsequent extraction by waterworks companies, led to a lowering of water levels, and the river was gradually canalised to maintain navigation.

The second approach [ 7, 8] is based on cross-linking p53 to genomic DNA, with subsequent extraction of the p53-bound DNA fragments by chromatin immuno-precipitation (ChIP).