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We validate our claims by subsequent experiments.
It was these auxiliary assumptions that were tested and eliminated as alternative explanations by subsequent experiments.
Even though the inferred spatial repartition along ecological gradients is rather a hypothesis in need to be confirmed by subsequent experiments in the laboratory, it has become evident that C. riparius and C. piger are ecologically not completely equivalent.
The observation that 363 MCP elicited lower titers of antibodies binding to native gp120 than 419 MCP or 426 MCP (Tables 1 and 2) was explained by subsequent experiments demonstrating that most of the antibodies induced by 363 MCP were directed against epitopes accessible in RCM gp120 in which S-S bonds are disrupted (Table 2).
We demonstrated by DNA microarray analysis that the categories of cell envelope and transport/binding proteins are the most actively regulated gene categories in the ΔrovA mutant, and this was validated by subsequent experiments showing that the cell membranes of the mutant bacteria were both structurally and functionally disrupted.
In most cases, the predictions of the models have been fully confirmed by subsequent experiments, as summarized in [ 43].
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This indicates that Def1 processing is a posttranslational event, a conclusion that was further supported by all subsequent experiments.
Cytokine Assays TNFα and IL-10 were quantified by cytometric bead array (BD) for initial PBMC stimulations, by ELISA for subsequent experiments (Quantikine ELISA; TNFα, IL-10 and TGFβ1) and by Meso Scale Discovery immunoassays for other cytokines (GMCSF, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8 and IL-12p70).
The identity of amplified products was initially confirmed by direct sequence analysis, and by Southern blotting in subsequent experiments.
Subsequent experiments by both men and other researchers over the intervening decades suggest that in a crisis, pointing at a bystander and giving her directions to ask help or complete a task can override the pernicious effect.
However, subsequent experiments by Denk, Herrmann and others have confirmed this equilibrium, albeit in specific circumstances.
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