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The genotypes of PR2, PR5 and PR7 were confirmed by subsequent analyses of progeny plants.
By subsequent analyses of off-target disruption within the CCR2 locus of the same cells, we detected disruption (3%) induced by plasmid-encoded ZFNs, whereas indels and mismatches were not evident after ZFN protein transduction.
However, silenced transgenes integrated into heterochromatin regions were not included in the study due to selection bias, as revealed by subsequent analyses of transgenic plants or cell lines generated without selection pressure [ 12, 14].
The group classification of legume bZIP genes based on their phylogenetic relationships was supported by subsequent analyses of gene structure, intron phases in the bZIP domain, MEME motif composition, DNA-binding specificity and dimerization patterns, which showed group-specificity.
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Reports from these studies have largely been disappointing, with numerous positive associations initially from analyses of small case control series being unconfirmed by subsequent analyses.
The appropriateness of this approach was indeed confirmed by subsequent analyses (vide infra).
Feed and fecal samples were dried, pooled on tank basis (applied for feces only) and homogenized by grinding before subsequent analyses of dry matter, nitrogen, energy, lipid and yttrium oxide.
Subsequent analyses of total RNA by RT-PCR, followed by cloning and sequencing of the amplification products, conclusively confirmed HSVd and GYSVd1 infections.
Subsequent analyses of metabolites by gas chromatography mass spectrometry (GC-MS) were performed as described elsewhere [ 108, 109].
Although the candidate protein profiles we have highlighted here are not constrained by a certain blood preparation type, subsequent analyses of larger sample cohorts might indeed unveil whether any of these two blood preparations is preferable.
Genes affected by the genetic background can be excluded in subsequent analyses of the disease-related changes in expression profiles.
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