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Pt/VC electrodes were contaminated by submersion in a SO2-containing solution made up of 0.2 mM Na2SO3 and 0.5 M H2SO4 for different periods of time, and held at 0.05 V (vs. RHE) in 0.5 M H2SO4 solutions in order to gain zero-valence sulfur (S0) poisoned electrodes.
First, all cadavers were immediately fixed postoperatively by submersion in a 10% formalin solution.
Salamanders were euthanised by submersion in a 10% solution of MS-222 according to IACUC protocols.
Cells were fixed by submersion in a citrate-acetone-formaldehyde solution at room temperature for 30 s, then washed with deionized water before application of a reaction solution containing α-napthyl acetate for 30 min at 37°C.
To form thermally denatured bovine serum albumin gels (TBSA), the precursor solution was neutralized to pH 7.4 by 2 M NaOH followed by submersion in a water bath at 80 °C for 2 h.
Mosquitoes were instantaneously killed in Eppendorf tubes by submersion in a dry ice/liquid nitrogen bath at 14 days post-blood meal and stored in RNAlater prior to RNA extraction.
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Seeds of E. acanthocarpum, donated by Jardín Botánico Viera y Clavijo (Gran Canaria, Spain), were surface sterilized by a brief immersion in 70% EtOH, followed by submersion in an aqueous solution of 5% (v/v) of commercial bleach for 25 min with gentle hand agitation.
All glassware used in the formation of SAMs was first cleaned by submersion in piranha solution, a mixture of 30% hydrogen peroxide and 95% concentrated sulfuric acid in the ratio 3 7 for at least 40 min. (Caution: Piranha solution is an extremely strong oxidizing agent which has been known to detonate spontaneously upon contact with organic material).
Crystals were frozen by submersion in liquid nitrogen after a few seconds incubation in cryoprotectant containing the above constituents supplemented with 25% ethylene glycol and 5 mM 5-FU.
Young leaves were collected, rapidly washed in double-distilled water, and immediately frozen by submersion in liquid Nitrogen, followed by a short-term storage (−80°C).
Larvae were washed twice to remove surface contamination and sterilized by submersion in 70% alcohol for approximately 3 min. Sterilized larvae were placed in a sterilized tissue culture dish.
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