Brecht, M., Schneider, M., Sakmann, B. & Margrie, T. W. Whisker movements evoked by stimulation of single pyramidal cells in rat motor cortex.
There is evidence that fMRI signal changes can be elicited in the parietal cortex by stimulation of single mechanoceptive afferent fibers at suprathreshold intensities for conscious perception.
Seventeen cytokine profiles were examined in PBMCs cultures obtained from 29 subjects by stimulation of single or different combinations of DPT, Hib, PCV7, DPT/Hib, DPT/PCV7, Hib/PCV7, and DPT/Hib/PCV7.
Conceptually, this is not limited to three muscles (like in our study) and potentially allows generation of complex functional motor maps of a whole hemisphere by stimulation of a single grid.
After a stable EPSP baseline period of at least 10 min, STDP was induced by repeated pairings of single presynaptic EPSP evoked by stimulation of Schaffer collaterals and single postsynaptic action potential elicited with the minimum somatic current pulse (1 1.8 nA, 3 ms) via the recording electrode.
ICWs were induced by focal mechanical stimulation of single astrocytes and the radius of calcium wave spread from the point of stimulation was measured before and after exposure to LDPBS (low-divalent PBS; containing nominally zero Ca2+ and 43 μM MgCl2, prepared by dissolving 1 mM MgCl2 and 1 mM EDTA in the Ca2+- and Mg+2-free DPBS), as previously described (Suadicani et al., 2006).
Mechanical stimulation of single cells by touching with glass pipettes (tip diameter 1 μm) increased the Ca2+i of the stimulated cell.
The GIY-YIG nuclease domain is also present in the Slx1 protein, a subunit of a eukaryotic yeast heterodimeric structure-specific endonuclease that is involved in the maintenance of rDNA copy number by stimulation of recombination of arrested replication forks via single-strand cleavage on the 3' side of the junctions [ 22, 23].
This has been cemented with evidence gathered from neuroimaging18,44, visual hallucinations induced by stimulation of the PFC45,46, impairment of conscious perception in macaques and human subjects following PFC lesions21,47,48,49,50, and more recently single neuron recordings11,51,52,53.
In two patients with hemifacial spasm (HFS), single-fiber EMG recordings in facial muscles demonstrated low jitter in the late responses produced by stimulation of peripheral branches to other facial muscles.
Finally, a word about models: it is impossible to elicit a spike by extracellular stimulation of a single compartment model i.e. a model cell whose membrane potential is uniform over its surface.
