Sentence examples for by stereological methods using from inspiring English sources
Total number of proliferating cells (BrdU+) and proliferating oligodendrocyte progenitor cells (BrdU/NG2+) was assessed separately in the SVZ, cortex and corpus callosum by stereological methods using the optical fractionator probe (Stereo Investigator Version 6, Micro Brightfield).
Vessel density was counted on CD34 and CD105 immunostained slides, according to conventional stereological methods using an unbiased Gundersen counting frame (Gundersen, 1978).
The stereological method used the physical disector, a volume probe comprising a pair of sections separated by a known distance, h (Sterio 1984; Gundersen 1986).
The density of this type of interneuron was calculated by stereological methods in six cases (Table 1), and determined along the complete anteroposterior length of both the CN and Put following a stereological protocol described elsewhere [39].
The number of motor neurons in each section was counted based on stereological counting methods using adjacent sections as a reference and look-up section, respectively (physical disector).
Then the planar gradation was transformed to the three dimensional gradation by stereological method.
Data obtained with stereological methods were used to normalize the data obtained with confocal microscopy to the whole DG using arithmetical proportion.
Stereological methods were used to count cells positive for Iba-1 microglial and DAPI in the TNC by an observer blinded to the experimental conditions.
For NeuN+IR cell counting, a stereological method was used to quantify cells within the THAL and ENT following NeuN immunostaining of brain sections using the Computer Assisted Stereological Toolbox stereological system as described previously (Crews et al., 2013).
Stereological analysis and quantification of neocortical and hippocampal intra-neuronal FL-αsyn and CC-αsyn immunoreactivity was conducted by the disector method using the Stereo-Investigator System (MBF Bioscience, Williston, VT) and the results were averaged and expressed as cell counts per 0.1 mm3.
The lungs were removed for determination of the metastasis burden by stereological analysis using Cavalieri's principle as described previously [29].
