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The effects of streptomycin (STM) on the development of antibiotic resistance in an aerobic-biofilm reactor was explored by stepwise increases in STM doses (0 50 mg L−1), over a period of 618 days.
After PCR, melting curves were acquired by stepwise increases in the temperature from 65 to 95°C to ensure that a single product was amplified in the reaction.
Human liver carcinoma cells BEL-74044) and human KB adenocarcinoma cells were selected by stepwise increases in cisplatin.
Markers that could not be ordered with a high confidence level were submitted for re-analysis by stepwise increases in the Lod score to > 9.0, forcing the linkage group to split into two or more groups, until satisfactory order with a high statistical confidence level was achieved for each resulting group.
The GLC4-Adr sub-line acquired resistance not only to doxorubicin but also to a wide range of other chemotherapeutic agents, by stepwise increases in doxorubicin concentrations in the culture medium (Zijlstra et al, 1987; De Jong et al, 1990; Meijer et al, 1991; Müller et al, 1994).
In order to assess gas exchange at comparable levels, we chose to perform a SI followed by stepwise increases in mPaw, which was measured continuously in the trachea using a pressure transducer fast enough to detect pressure changes at 6 Hertz.
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In the event of severe respiratory failure, recruitment maneuvers by stepwise increase in the mean airway pressure were applied.
In the bioreactor validation the maximum temperature was determined by stepwise increase in temperature until stable growth was no longer possible.
CS-1/ER and CS-1/PR were selected from the parental cell line, CS-1, by exposure to stepwise increases in ET-743 or PM00104 concentrations for a period of one year.
Stepwise increases in pD were achieved by the addition of NaOD (1 m or 200 m m in D2O).
In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica.
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