Sentence examples for by stepwise increase of from inspiring English sources
Meat samples were first dried in oven at 105°C for 24 hours and then digested in the Muffle furnace by stepwise increase of the temperature up to 550°C till the white ash formed.
The first dimension (IEF) was performed in the IPGphor Isoelectric Focusing System (Pharmersham) by stepwise increase of the voltage as follows: 0 V–500 V for 2 h, 500 V for 5 h, 500 V–3500 V for 3 h and finally 3500 V continuing until the total volt-hours reached 45 KVh.
Productivity was improved by stepwise increase of the methotrexate (MTX) concentration and two steps of subcloning by limiting dilution.
One mL fractions were eluted with equilibrating buffer, followed by stepwise increase of NaCl concentration from 0.3 M to 0.9 M NaCl.
Absorbed protein was eluted into 12 fractions (1 ml each) by stepwise increase of NaCl concentration in buffer C. We used 0.6 M NaCl eluate as the purified DNA-PK holoenzyme.
Thereafter, PEEP was set at 20 cmH2O and the lungs were recruited by stepwise increases of the driving pressure up to 30 cmH2O (time = T20/30).
TNF-α significantly shifted the Ca2+ diameter relation to the right, as assessed by stepwise increasing of extracellular Ca2+ (0–1.5 mmol/l) in depolarized skeletal muscle resistance arteries.
To achieve high-level expression, amplification of the transgenes was conducted by stepwise increasing of the Zeocin concentration in case of P. pastoris and the MTX concentration for CHO cells according to established standard protocols.
The findings of an HFOV initiation protocol by stepwise increases of CDP can therefore only be compared with the used lung recruitment strategy by PCV with PEEP increases coupled to a constant inspiratory pressure amplitude (PEEP + 20 mbar).
The LBNP protocol consisted of 5-minute baseline measurements in the supine position followed by stepwise increases of negative pressure from 0 to -20, -40, -60, -80 and 0 mmHg.
The primary objective of this study was to asses whether a recruitment procedure of the lung, at initiation of HFOV by stepwise increases of continuous distending pressures, impairs the hemodynamics and organ blood flow in lung-injured animals.
