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In the present study, a two-stage glucose-feeding strategy by stepwise decrease in feeding rate was proposed to adaptively respond to variation in glucose consumption rate throughout the feeding phase.
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Then, the cells were adapted to serum-free medium by stepwise decreasing the serum concentration by 50% every three passages until the serum concentration was reduced below 0.1%.
The assembly was further refined using "Single-to-End" and "End-to-End" merging by stepwise decreasing of assembly stringency of Sulston score cutoff values (down to 1× 1×-1×).
GC electrodes were polished with aqueous alumina slurries (Al2O3) by stepwise decreasing the particle size (1 to 0.05 μm) on wet microcloth pads (Buehler, Lake Bluff, IL, USA) using Milli-Q water.
The highest iturin A concentration of 1.12 g/L was obtained by adopting the proposed two-phase feeding strategy by a stepwise decrease in the feeding rate.
The protocol consists of a fast recruitment maneuver followed by a stepwise decrease of CDP.
The LVOP consists of a single CDP recruitment maneuver during uninterrupted HFOV, followed by a stepwise decrease of the CDP.
Preclinical and clinical trials have been presented recently that used a recruitment maneuver with a high CDP, followed by a stepwise decrease of the airway pressure.
A stepwise decrease in PNA binding by preneoplastic D2HAN cells and subsequent tumours arising from those hyperplastic lesions was observed.
Inhibition of Sirt1 by increasing concentrations of nicotinamide led to a stepwise decrease of viable cells as depicted in Figure 5.
We demonstrated that continuous and significant enhancement of iturin A production could be achieved by a novel two-stage glucose-feeding strategy with a stepwise decrease in feeding rate.
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