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The pH value of Cd precursor needs to be adjusted to 9.5 by stepwise addition of NaOH at 4 °C.
All the elements were assembled by stepwise addition of three cassettes for which the design was made de novo.
The chemical synthesis of the peptide was performed with solid phase method by stepwise addition of protected amino acids to a growing peptide chain.
Then the concentration of NaCl in the solution was slowly increased to 0.1 M in a 6-h time window by stepwise addition of 1 M NaCl aqueous solution.
The PQE and PSE films doped with FeCl3 showed considerable conductivities, 9-7×10−3 and 0.4-0.2 0.4-0.2eS cmtively. The polymerespectivelyand films were oxidized (doped) by stepwise addition of FeCl3 and characterized by electronic absorption spectra.
We also investigated the effect of incrementally activating respiration by stepwise addition of increasing concentrations of ADP, allowing H+ flux through Complex V and thereby harnessing the membrane potential for ATP production and reducing H2O2 production.
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CaCl2 was then cautiously re-introduced by stepwise additions of CaCl2 to reach an approximate concentration of 1.25 m m by centrifuging the preparation at each Ca2+ reloading stage to remove dead or swollen cells.
The cell pellets were washed three times with fresh LB medium and then acid-digested by stepwise additions of 70% (v/v) nitric acid, 30% (v/v) hydrogen peroxide, and concentrated HCl at 95°C adapting EPA method 3010A [ 26].
The free R. oryzae lipase F-APl5 (Amano) catalyzed the methanolysis of soybean oil which reached 80 wt.% yield of fatty acid methyl esters (FAME) by stepwise additions of methanol to the reaction mixture in the presence of 4~30 wt.% water [ 13].
Tissue extraction is usually performed by the cooled homogenization of tissue, followed by the stepwise addition of reagents and solvents of differing polarities such as perchloric acid or methanol chloroform mixtures.
Asymmetric and mikto-arm stars were synthesized by the stepwise addition of macro-radicals or of anionic polymer chains onto C60.
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Justyna Jupowicz-Kozak
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