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To realize whole-organ replacement therapy as next-generation regenerative medicine, it is expected that fully functional bioengineered organs can be reconstructed using an in vitro three-dimensional (3D) bioengineered organ germ and organoids by stem cell manipulation and self-organization.
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Current and future research aims to identify key stem cell regulators and methods for in vitro and in vivo stem cell manipulation including genome editing.
In order to mimic this niche, nano biomaterials are being precisely engineered to enable specific stem cell manipulation and interaction.
This stem cell manipulation approach is fast, straightforward and safe, does not require genetic alteration of the cells, and should be generalizable to multiple cell types.
CP designed vectors and performed embryonic stem cell manipulation.
Development, cancer, neurodegenerative and demyelinating diseases, injury, and stem cell manipulations are characterised by alterations in cell number.
CDKIs also constitute potential targets for therapeutic stem cell manipulations [20].
The prepared paper scaffolds are compatible with general stem cell culture and manipulation techniques.
Nanoparticles are a promising platform for stem cell study and manipulation [16].
Currently, most advanced methods for manipulation of hPSC fate involve recapitulating developmental processes by mimicking stem cell niche-associated signals.
A hallmark of regenerative medicine is the clinical use of stem cells, either by manipulation of endogenous progenitor populations in situ, or by transplantation of stem cells (autologous or allogeneic).
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