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The ODEs were analyzed by standard techniques.
The products were characterized by standard techniques.
Coding regions, intron-exon boundaries, and untranslated regions were sequenced by standard techniques.
Western blotting was performed by standard techniques.
Specific binding was visualised by standard techniques.
Pancreatic islets were isolated by standard techniques, with minor adaptations.
Single cell suspensions from spleens were prepared by standard techniques.
Anthropometric measurements were obtained by standard techniques as described in Weiner and Lourie [22].
Total RNA was collected by standard techniques [33] and converted to cDNA.
Tissue embedding and haematoxylin and eosin staining were performed by standard techniques.
Fasting triglyceride, cholesterols, homocysteine, urine albumin, and urine creatine were measured by standard techniques.
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