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This is especially true for large highly polymorphic genes such as USH2A that often have mutations that are difficult to identify by standard sequencing techniques.
We hypothesized that reinfection rates following treatment may be overestimated by standard sequencing techniques due to a lack of detection of varying dominance of minority variant strains present at the onset of infection.
Assuming that these results were favorable, etravirine could also represent an option for subjects with primary, transmitted NNRTI resistance, especially since it is not always detected by standard sequencing techniques.
The nucleotide sequence was confirmed in each expression construct by standard sequencing techniques (Quintara Biosciences, Berkeley, CA) The AtPum2 PUM-HD coding sequence in pGEX-6P-1 was expressed in E. coli strain BL21 DE3) as a fusion to the carboxyl-terminus of glutathione S-transferase (GST).
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The combination of next-generation sequencing and metagenomic analysis identified a novel paramyxovirus; the virus genome was completely characterized by use of standard sequencing techniques.
Structural alignment is a valuable tool for the comparison of proteins with low sequence similarity, where evolutionary relationships between proteins cannot be easily detected by standard sequence alignment techniques.
PCR products were analyzed by standard sequencing.
The results were confirmed by standard sequencing.
One application of this technology is ultra-deep pyrosequencing (UDPS), which allows identification of rare genetic variants and minority drug resistance mutations, which are not detectable by standard genotypic sequencing techniques [12], [17], [18], [19].
In the second part, we corroborated the results (obtained by the HRM method) using the standard Sanger sequencing technique.
This level of genomic variability is unexpected considering the difficulty of distinguishing among strains using standard molecular techniques and underscores the resolution provided by massive sequencing techniques.
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