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All constructs were verified by standard sequencing methods.
All plasmid constructions were verified by standard sequencing methods (IRIC genomic platform, Montréal, Canada).
However, most (99%) of the drug resistance could remain 'hidden' as minority-resistant variants that are not detectable by standard sequencing methods.
In the absence of the pressure of ART, majority-resistant strains of HIV tend to revert to become minority-resistant variants that appear to be exclusively wild-type and not detected by standard sequencing methods.
Nucleotide sequences of cDNA clones were determined using automated DNA sequencing machines by standard sequencing methods.
NGS can detect variants at low frequencies, which would go undetected by standard sequencing methods [ 7].
Similar(53)
Direct mutation detection was achieved by either minisequencing or standard sequencing methods.
PCR products were analyzed by standard sequencing.
The results were confirmed by standard sequencing.
We then developed high-fidelity polymerase chain reactions to produce amplicons using 1 mL of blood from each subject; amplicons were hybridized to the chip, and nucleotide calls were validated by standard capillary sequencing methods.
All constructs were verified using standard DNA sequencing methods.
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