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We sought to determine whether deletions and duplications are a common cause of LGMD that have been routinely missed by standard sequencing approaches.
PCR products were analyzed by standard sequencing.
All constructs were verified by standard sequencing methods.
Ultra-deep sequencing identified all DRMs at levels ≥20% that were detected by standard sequencing.
All plasmid constructions were verified by standard sequencing methods (IRIC genomic platform, Montréal, Canada).
The high-abundance mutations that were not detected by standard sequencing were at levels between 20 to 30.5%.
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Structural alignment is a valuable tool for the comparison of proteins with low sequence similarity, where evolutionary relationships between proteins cannot be easily detected by standard sequence alignment techniques.
Raw sequences are trimmed, aligned and analysed by standard sequence analysis tools [ 8, 9].
The short-listed targets were compared to these anti-targets by standard sequence analysis.
Changes in denaturing high-performance liquid chromatography elution profiles were verified by standard sequence analysis.
From 30%to40%0% of cDNAs from non-model organisms such as conifers could not be annotated by standard sequence similarity searches like BLAST [ 15, 16] or HMMER [ 13].
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