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After transillumination of the abdominal wall PEG catheter Flocare Ch18 (Nutricia) was inserted by standard pull method.
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The mechanical strength of coatings was evaluated by standard pull-off test.
LES, esophageal body and UES pressures were studied according to standard pull-through method in response to 5 mL water boluses.
DNA was extracted by standard salt method.
Cleavage by thrombin (Sigma) was performed by standard method.
Total protein concentration was determined by standard method.
Chemical data were determined in triplicates by standard oceanographic methods.
Genotypic analyses were performed by standard PCR methods.
All constructs were verified by standard sequencing methods.
Organisms were identified by standard microbiological methods.
Creatinine was determined by standard laboratory methods.
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