Sentence examples for by standard protocols using from inspiring English sources

Recombinant His-C3G fusion protein was purified form Echerichia coli (BL21 (DE3)) bacterial lysates by standard protocols using Ni-NTA agarose (Qiagen) and subsequently used for immunization of guinea pigs.

Genomic DNA was extracted by standard protocols using QIAmp DNA Mini kit (QIAGEN) according to the manufacturer's recommendations.

Restriction analysis and southern hybridization were performed by standard protocols, using probes labelled with digoxigenin-11-dUTP (Roche Diagnostics, Milan, Italy).

The stable HeLa cell line for EYFP-SUMO1 was generated and maintained by standard protocols using 400 μg/ml G418 as selective marker (Trinkle-Mulcahy et al. 2007).

Radiochemical yields were determined by standard protocols using efficiencies calculated with a NIST traceable, multiline α-spectrometry standard source (E&Z 91005 or Analytics 59956-121).

Agarose gel electrophoresis was performed by standard protocols using 1× TAE buffer; DNA fragments were purified using the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare), according to the manufacturer's instructions.

Tissue sections were pretreated by standard protocol using the VP2000™ slide pretreatment instrument (Abbott Molecular).

HRCT examination was performed by standard protocol using a CT 64 E light Speed VCT power scanner with a rotation tube with a scanning time of 0.65 seconds.

The multilineage differentiation potential of human MSC (n = 3, P3) was analyzed by applying standard protocols used by Pittenger et al. [ 1].

Cell cycle analysis was performed by flow cytometry following standard protocols used in our laboratories [26], [50].

The tissue were sliced and stained according to standard protocols used by American Histolabs, Gaithersburg, MD.