Sentence examples for by standard protocols in from inspiring English sources

Bioassays for IFNγ were done by standard protocols, in which we incubated 40 µl of culture supernatant with 20 µl (5000 cells/well) of WEHI-279 indicator cells (ATCC, Rockville, MD) in the presence and absence of anti-IFNγ to measure IFNγ and pulsed cultures with [3H]-thymidine at 24 h and harvested 24 h after.

In brief, we sequenced biological triplicates of polyadenylated RNA from T. cruzi epimastigotes and in vitro differentiated metacyclic trypomastigotes by standard protocols in the SOLiD platform.

Immunoblotting and quantitative polymerase chain reaction were carried out by standard protocols (details in Supplementary Material; n = 3 independent experiments in triplicate).

All screening was done at 10 μM J147 by various contract research organizations (CROs), including MDS Pharma Services (King of Prussia, PA, USA), Ricerca, now Panlabs (Concord, OH, USA), Ambit (La Jolla, CA, USA), Caliper (Hopkinton, MA, USA) and NovaScreen Biosciences (Hanover, MD, USA) by standard protocols described in their catalogs.

Whole-mount in situ hybridization was done by standard protocols [86], [87].

Embryo fixation, antibody staining and in situ hybridization were carried out by standard protocols [10].

DNA was isolated by standard protocols.

Mitochondrial isolation was performed by standard protocols [28], [29].

The in vitro culturing and synchronization of P. falciparum (isolate FCBR) was carried out by standard protocols [57], [58].

Bacteria were grown by standard protocols.

Western blotting was performed by standard protocols.