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The synthetic leachates were prepared by standard protocols and two types of modified procedures with much higher dilution ratios that resulted in reduced ionic strength.
Waist size and hip size were measured by standard protocols and waist-to-hip circumference ratio was created.
Plasmid DNA was prepared by standard protocols, and cloned inserts were bidirectionally sequenced with M13 forward and M13 reverse primers using the BigDye Terminator v3.1 chemistry (Applied Biosystems, Foster City CA).
BP was measured by standard protocols, and M-mode echocardiographic parameters were determined by ultrasonography.
Genomic DNA was isolated by standard protocols, and equal amounts of DNA were digested with HinfI and RsaI.
Genomic DNA was extracted from peripheral blood lymphocytes by standard protocols, and teeth were also obtained where available.
Similar(48)
Clinical specimens were prepared by standard protocol and inoculated for culture on Lowenstein-Jensen slants and in liquid media throughout the study period.
The sequencing was performed on ABI 310 automatic sequencer using Big Dye Terminator v 1.1 Cycle Sequencing kit (Applied) by standard protocol and primers from the second PCR.
Following PCR, the products were run in 1% agarose, then cut, blunted by standard protocol and cloned into pJET 1.2 vector.
p{UAS-Ras85D V12C40} and p{UAS-ERK D334N} were engineered by site-directed mutagenesis from cDNA from w, and transgenic insertion lines were obtained by standard protocols (Brand and Perrimon, 1993).
Tissue was stained by standard protocols with hematoxylin and eosin as well as Masson's trichrome to detect damage by cellular infiltration and fibrosis.
More suggestions(15)
by various protocols and
by standard accounts and
by strict protocols and
by standard methods and
by standard dilutions and
by standard codes and
by different protocols and
by experimental protocols and
by standard technologies and
by standard therapies and
by standard techniques and
by nonstandardized protocols and
by nonstandardised protocols and
by local protocols and
by standard equations and
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