Sentence examples for by standard protocols according from inspiring English sources

The protein concentrations were tested by standard protocols, according to the suggestion of the manufacturers.

The next day, cells were transfected with 15 nℳ miRNA precursors or siRNA against eEF1A2 using RNAiMAX (Invitrogen) by standard protocol according to manual.

Total RNA was isolated with TRIzol reagent by a standard protocol according to the manufacturer's protocol (Invitrogen, Carlsbad, CA, USA).

Silvio Gutkind (National Institutes of Health, USA) kindly provided the construct of Rac1 Q61L/pcDNA 3. The HA-tagged mRIN2 construct was generated by standard PCR protocols according to the Taq polymerase manufacturer's instructions (Fynnzymes), using mRIN2/pCMV-Sport6 (Invitrogen) as template and phCMV-2 (Genlantis) as host expression vector.

Genomic DNA was extracted by standard protocols using QIAmp DNA Mini kit (QIAGEN) according to the manufacturer's recommendations.

DNA was isolated by standard protocols.

RT-PCR was performed by standard protocol.

Plant protein extracts and protein gel blots were carried out by standard techniques, according to protocols described in the Additional file 13.

Preparation of chromosomal and plasmid DNA, and transformation were carried out by standard procedures or according to supplier protocols (Qiagen, Frederick, MD, USA).

For light and fluorescence microscopy, embryos were fixed chemically (in 3,7% formaldehyde) or physically (by boiling) according to standard protocols [27].

Height (cm) and weight (kg) were measured by nurses according to standard protocols.