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The prodrug was synthesized by standard procedures using dipalmitine as a carrier and the structure was confirmed by spectral analysis.
The SAXS intensity was recorded on a 2D-CCD detector, corrected for background and scattering of the empty capillary, and converted into absolute units by standard procedures using a standard of known scattering intensity.
PCR reaction conditions were performed by standard procedures using pfu turbo polymerase (stratagene).
Primers were designed by standard procedures using Clone Manager version 9.0 software Sci-Ed Softwaree). Sci-Ed Software
Chromosomal DNA from cells and bacteria was prepared by standard procedures using phenol/chloroform extraction.
Northern blotting was done by standard procedures using randomly primed alpha-dCT32P labeled probes.
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Bronchoscopy with bronchoalveolar lavage was performed by standard procedure using a flexible fibreoptic bronchoscope (Olympus p45, Tokyo, Japan) wedged into involved segmental bronchi.
Genomic DNA of all women was extracted from peripheral blood leukocytes by standard procedure using commercially available kit (Qiagen FlexiGene DNA kit, Qiagen GmbH, Hilden, Germany).
Bacterial testing was by SNBTS standard procedure using the BacT/Alert 3D system (BioMérieux, Basingstoke, UK).
Genomic DNA was isolated by a standard procedure using NucleoSpin PlantII (Macherey-Nagel GmbH & Co. KG, Dueren, Germany).
Western blots were prepared by standard procedures, probed using appropriate dual immuno-staining and visualised by chemiluminescence (ECL plus; GE Healthcare) according to the manufacturer's instructions.
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by standard approaches using
by conventional procedures using
by fitting procedures using
by standard procedures according
by standard protocols using
by standard techniques using
by enzymatic procedures using
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by standard criteria using
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by microanalytical procedures using
by experimental procedures using
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